Metagenome sequencing with MR DNA

Blog

  • Sample Submission Instructions – MR DNA

    📦 Sample Submission Instructions – MR DNA

    Simple, Clear Guidelines for Submitting Your Samples for Sequencing

    At MR DNA, we’ve made the sample submission process quick, easy, and reliable. Whether you’re sending DNA, RNA, swabs, stool, soil, or environmental samples, follow these guidelines to ensure your materials arrive safely and ready for processing.

    We handle thousands of samples annually — and we work hard to ensure every one is treated with care and precision.

    🧬 Sample Types Accepted

    We accept a wide range of sample types for microbiome, metagenomics, genome, transcriptome, and virome analysis:

    • 🧫 DNA & RNA (extracted by client or MR DNA)

    • 💩 Stool and fecal material (fresh or frozen)

    • 🧪 Oral, vaginal, skin swabs

    • 🌱 Soil, water, sediment, and plant root samples

    • 🧬 Cultured isolates (bacteria, fungi, viruses)

    • 🧫 Tissue, urine, saliva, and environmental wipes

    📋 General Submission Guidelines

    ✅ Label Clearly

    • Label tubes with sample ID that matches your submission form.

    • Use permanent ink or printed labels — no handwritten stickers that may smudge.

    ✅ Use Proper Containers

    • For extracted nucleic acids: Use screw-cap microcentrifuge tubes (1.5 mL or 2.0 mL preferred).

    • For swabs: Use DNA/RNA-free tubes or commercial collection kits.

    • For fresh stool or soil: Use sterile tubes with tight sealing caps.

    ✅ Volume & Concentration

    Sample Type Minimum Volume Minimum Concentration
    DNA 20–30 µL ≥10 ng/µL (ideally 20+)
    RNA 20–30 µL ≥20 ng/µL, RIN >7 preferred
    Swabs 1 swab/tube Dry or in DNA/RNA shield
    Soil/Feces ~200 mg In sterile tube

    ✅ Shipping

    • Use insulated packaging with ice packs or dry ice for temperature-sensitive samples.

    • Ship Monday–Wednesday to avoid weekend delays.

    • Overnight shipping is strongly recommended.

    📤 Include This with Your Shipment

    • 📝 Sample Submission Form (provided at time of quote or via our website)

    • 🧾 Quote or PO number (if applicable)

    • 🧬 Contact info – email, phone, institution

    • 📦 Clearly labeled box or envelope

    📮 Ship To:

    MR DNA – Sample Receiving
    1301 Memorial Drive
    Suite B
    Shallowater, TX 79363
    Phone: 1-806-789-7984
    📧 Email: info@mrdnalab.com

    🔄 DNA Extraction Service

    Don’t have access to a lab? We can extract DNA or RNA from your samples:

    • Stool, swabs, soil, and more

    • Host DNA removal options available

    • Just indicate on your submission form if extraction is required

    📞 Questions About Sample Prep?

    Not sure if your samples are ready? Contact us — we’re happy to help.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    📥 Contact Form: Submit a question

    🔑 SEO Keywords Embedded:

    • sample submission for sequencing

    • DNA RNA shipping instructions

    • stool and soil microbiome samples

    • microbiome sample preparation

    • MR DNA sample guidelines

    • how to submit samples for metagenomics

  • Plant & Rhizosphere Microbiome Sequencing – MR DNA

    🌱 Plant & Rhizosphere Microbiome Sequencing – MR DNA

    Unlock Root Health and Soil Microbial Diversity with Precision Sequencing

    At MR DNA, we provide advanced sequencing solutions for studying the plant-associated microbiome, with a focus on the rhizosphere, phyllosphere, and soil-root interface. Our services help you understand microbial influences on plant growth, disease resistance, nutrient cycling, and agricultural sustainability.

    With affordable pricing, fast turnaround, and expert bioinformatics, MR DNA is your partner for microbial ecology and agri-biotech research.

    🧬 What We Sequence

    Sample Type Application Areas
    Rhizosphere Soil Root-microbe interactions, fertilizer response
    Root Surface (Endorhizosphere) Plant-microbe symbiosis, pathogen resistance
    Bulk Soil Baseline microbial communities, agricultural amendments
    Plant Tissue Endophytic bacteria/fungi in stems, leaves, seeds
    Phyllosphere (leaf) Microbial populations on above-ground plant surfaces

    🔬 Available Sequencing Options

    • 16S rRNA Amplicon Sequencing – Bacterial and archaeal profiling

    • ITS Sequencing – Fungal and yeast communities

    • Shotgun Metagenomics – Full community structure and functional gene analysis

    • Virome Sequencing – Detection of soil- and plant-associated viruses

    • Metatranscriptomics (optional) – Active gene expression of root microbiomes

    📊 Expert Bioinformatics Included

    Every project comes with full in-house analysis:

    • ✅ Taxonomic classification (SILVA, Greengenes, UNITE)

    • ✅ Alpha & beta diversity (richness, Shannon, Bray-Curtis)

    • ✅ Comparative analysis (treatment vs control, genotype, time series)

    • ✅ Functional pathways (KEGG, SEED, nitrogen cycling, P solubilization)

    • ✅ Soil health and disease-suppressive profile indicators

    • ✅ Visualizations: stacked bar plots, heatmaps, PCoA, LEfSe

    🌾 Who We Serve

    • Agricultural researchers

    • Soil ecologists and microbiome labs

    • Crop scientists and plant breeders

    • Fertilizer and biostimulant developers

    • Sustainable farming consultants

    • Cannabis, hemp, and specialty crop producers

    💰 Affordable and Flexible

    • 📦 Submit soil, root, or plant tissue samples

    • 🔬 DNA extraction available or accept your prepped DNA

    • 🧪 Library prep, sequencing, and analysis included

    • 📈 Flat-rate pricing and bulk discounts

    • ⚡ Turnaround time: 2–3 weeks typical

    📞 Start Your Soil or Plant Microbiome Project with MR DNA

    Whether you’re profiling soil for a field trial or testing microbial additives in a greenhouse, we’re ready to help.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • plant microbiome sequencing

    • rhizosphere microbial profiling

    • root-associated microbiome

    • soil bacteria and fungi analysis

    • agricultural microbiome lab

    • microbial inoculant testing

    • MR DNA soil and crop microbiome services

  • Probiotic & Fermentation Microbiome Sequencing – MR DNA

    🧃 Probiotic & Fermentation Microbiome Sequencing – MR DNA

    Verify, Improve, and Discover with Precision Microbial Profiling

    At MR DNA, we offer targeted and shotgun sequencing services to support the growing fields of probiotics, fermented food microbiomes, and microbial product development. Whether you’re validating strain identity, optimizing a fermentation process, or profiling microbial stability, we provide accurate, affordable sequencing with expert analysis and fast turnaround.

    We work hard to help you deliver safe, effective, and scientifically supported microbial products.

    🧬 What We Sequence

    Sample Type Applications
    Probiotic Formulations Validate strain content, confirm labeling, detect contaminants
    Fermented Foods & Beverages Microbial profiles of kombucha, kefir, yogurt, sauerkraut, cheese, vinegar, beer
    Starter Cultures Batch QA, taxonomic confirmation
    Environmental Swabs Production line cleanliness, facility validation
    Gut Microbiome Probiotics Colonization studies, response tracking

    🔬 Available Sequencing Options

    • 16S rRNA Amplicon Sequencing – Bacterial genus/species ID

    • ITS Sequencing – Fungal/yeast profiling

    • Shotgun Metagenomics – Comprehensive community & functional profiling

    • Whole Genome Sequencing (WGS) – Strain typing and genetic fingerprinting

    • Virome Analysis – For phage discovery or viral load quantification

    📊 Bioinformatics for Industry & Research

    Our in-house analysis includes:

    • ✅ Microbial identification at genus/species level

    • ✅ Diversity metrics and comparative community profiling

    • ✅ Detection of spoilage organisms or contamination

    • ✅ Functional analysis: metabolic pathways, antimicrobial genes

    • ✅ Time-point comparisons or batch tracking

    • ✅ Custom reports for marketing, publication, or regulatory filing

    🧠 Use Cases We Support

    • Product development & strain selection

    • Label claim verification

    • Competitive market analysis

    • Shelf-life and stability studies

    • Pre/post digestion microbial response

    • Regulatory documentation support

    We work with startups, research teams, and established commercial brands.

    💰 Flat Pricing. Fast Turnaround.

    • 🔬 Full-service sequencing from extraction to analysis

    • 💡 Support for single batches or full product pipelines

    • 📈 Rapid delivery: most projects in 2–3 weeks

    • 💬 Direct access to our scientific team

    📞 Bring Your Microbial Products to Life with MR DNA

    Ready to optimize, validate, or explore your microbial products? We’re ready to help.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • probiotic sequencing services

    • fermentation microbiome analysis

    • fermented food microbial testing

    • yogurt and kombucha microbiome lab

    • microbial strain identification

    • shotgun metagenomics for probiotics

  • Microbiome Sequencing – MR DNA

    🧫 Clinical Microbiome Sequencing – MR DNA

    Accurate, Affordable Microbiome Profiling for Clinical Research & Diagnostics

    At MR DNA, we support hospitals, labs, and research institutions with clinical-grade microbiome sequencing and analysis. Whether you’re studying gut health, skin conditions, vaginal microbiomes, or infectious disease, we provide fast, reliable data with expert bioinformatics and clear, publication-ready reports.

    Our team works hard to ensure you get results you can trust — on time and within budget.

    🧬 What We Offer for Clinical Microbiome Studies

    We offer full-service sequencing for a range of clinically relevant sample types:

    Sample Type Applications
    Stool / Gut Microbiome GI health, IBD, IBS, dietary interventions, probiotics
    Vaginal Microbiome BV, STI risk, fertility, microbiota balance
    Oral Microbiome Periodontal disease, oral probiotics, systemic links
    Skin Microbiome Eczema, acne, psoriasis, wound healing
    Urine, Swab, Tissue UTI studies, cancer microbiome, surgical site infection
    Clinical Isolates Pathogen genome sequencing, resistance profiling

    🔬 Supported Sequencing Technologies

    • 16S rRNA (V1–V2, V3–V4, V4) – Bacterial community profiling

    • ITS sequencing – Fungal/yeast identification

    • Shotgun Metagenomics – Species-level taxonomy + functional profiling

    • Virome sequencing – Viral community detection and pathogen discovery

    • Whole genome sequencing – For clinical isolates (bacteria, fungi, viruses)

    📊 Bioinformatics for Clinical Research

    Every project includes robust bioinformatics tailored for clinical relevance:

    • ✅ Diversity metrics (Shannon, Simpson, beta diversity)

    • ✅ Pathogen detection and abundance tracking

    • ✅ Functional pathway analysis (HUMAnN, KEGG)

    • ✅ Antibiotic resistance and virulence gene detection

    • ✅ Comparative analysis (before/after treatment, healthy vs diseased)

    • ✅ Clean, editable reports for publications or regulatory review

    ✅ Designed for Clinical & Translational Research

    We support:

    • Academic hospitals and teaching labs

    • Clinical trials and investigational studies

    • Probiotic and medical device companies

    • Diagnostic test developers

    • Translational researchers and physicians

    MR DNA adheres to strict sample handling and documentation standards. HIPAA compliance and de-identified workflows available.

    💰 Transparent Pricing. No Compromise.

    • 📦 Flat-rate pricing per sample with analysis included

    • 🧠 Direct access to our scientific team for study design

    • ⚡ Turnaround: typically 2–3 weeks

    • 📈 Scalable for small pilot studies or full trials

    📞 Start Your Clinical Microbiome Project Today

    We’re ready to support your IRB-approved study, commercial research, or clinical pilot.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • clinical microbiome sequencing

    • gut and vaginal microbiome analysis

    • oral microbiome research

    • microbial diagnostics lab

    • pathogen detection by NGS

    • microbiome clinical trials

    • MR DNA microbiome services

  • Bioinformatics & Biostatistics Services – MR DNA

    📊 Bioinformatics & Biostatistics Services – MR DNA

    Expert Analysis You Can Trust – Included with Every Sequencing Project

    At MR DNA, we don’t just sequence — we help you understand your data. Our in-house team of bioinformaticians and biostatisticians works hard to transform raw reads into publication-ready insights, using validated tools and custom workflows tailored to each project.

    From microbial diversity to gene expression and pathway analysis, we provide accurate, actionable results — backed by real scientists and clear reports.

    🧬 Included with Every Project

    Every sequencing project at MR DNA includes standard bioinformatics analysis at no extra charge. Depending on the service type, this may include:

    🔹 Microbiome Projects (16S, ITS, 18S)

    • Quality filtering and demultiplexing

    • OTU or ASV clustering (QIIME2, DADA2)

    • Taxonomic classification (SILVA, UNITE, Greengenes)

    • Alpha & beta diversity metrics

    • Visualizations: heatmaps, bar plots, stacked plots, PCoA, rarefaction

    🔹 Shotgun Metagenomics & Virome

    • Host read removal (optional)

    • Taxonomic profiling (Kraken2, MetaPhlAn, Kaiju)

    • Functional annotation (HUMAnN3, KEGG, SEED, eggNOG)

    • Antibiotic resistance & virulence gene identification (CARD, VFDB)

    • Interactive outputs (Krona plots, pathway charts)

    🔹 Whole Genome & Transcriptome

    • Genome assembly (SPAdes, Flye, etc.)

    • Gene annotation (Prokka, RAST, NCBI pipeline)

    • RNAseq: differential expression (DESeq2, edgeR)

    • Full Iso-Seq transcript isoform mapping

    • Pathway enrichment (GO, KEGG)

    📈 Optional Advanced Services

    Need deeper analysis or custom comparisons? MR DNA offers add-on services including:

    • 🔍 LEfSe, PERMANOVA, ANOSIM, and pairwise testing

    • 🧠 Multi-group and multi-timepoint designs

    • 🧪 Strain-level or phylogenetic tree construction

    • 🧬 Pan-genome analysis and comparative genomics

    • 🗂️ Submission-ready GenBank file formatting

    • 🖼️ Customized figures for publication or grant submission

    🧠 Real Scientists. Real Support.

    Unlike automated pipelines, our team is available to:

    • Answer your questions

    • Review your results with you

    • Provide PDF summaries or editable data tables

    • Help you interpret unusual or unexpected findings

    • Support you through publication and peer review

    We work with clients in academia, clinical research, agriculture, industry, and beyond.

    💰 Included with All Projects – Or Available à la Carte

    • Most analysis is included at no extra cost

    • Custom bioinformatics is quoted based on sample count and complexity

    • Fast turnaround — usually 2–5 days after sequencing

    📞 Let’s Talk About Your Analysis Needs

    Whether you already have sequencing data or need full-service support, MR DNA is ready to help.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • bioinformatics analysis service

    • microbiome data analysis

    • shotgun metagenomics bioinformatics

    • RNAseq data interpretation

    • statistical analysis for sequencing

    • differential gene expression

    • MR DNA biostatistics support

  • PacBio HiFi Long-Read Sequencing – MR DNA

    🔬 PacBio HiFi Long-Read Sequencing – MR DNA

    Accurate, Full-Length Sequencing for Genomes, Amplicons, and Transcripts

    At MR DNA, we offer advanced long-read sequencing using the PacBio Sequel IIe platform, delivering highly accurate HiFi reads ideal for complete genome assemblies, long amplicons, and full-length transcript isoforms. Whether you’re sequencing bacteria, fungi, viruses, or transcriptomes, our long-read workflows deliver deep biological insight with affordable pricing and expert analysis.

    We work hard to make long-read sequencing accessible for any project.

    🧬 What Is PacBio HiFi Sequencing?

    PacBio HiFi reads combine long read lengths (10–25 kb) with Q20+ base accuracy (99.9%), allowing you to:

    • ✅ Assemble complete genomes and circular chromosomes

    • ✅ Sequence full-length amplicons with no need for overlap stitching

    • ✅ Detect structural variants, gene duplications, and mobile elements

    • ✅ Perform isoform-level transcriptomics (Iso-Seq)

    • ✅ Resolve complex or repetitive regions that short reads can’t

    🧪 MR DNA Long-Read Sequencing Workflow

    Step Description
    Sample QC DNA or RNA integrity check (Bioanalyzer or TapeStation)
    Library Prep SMRTbell library construction for HiFi reads
    Sequencing PacBio Sequel IIe (HiFi CCS reads)
    Data Delivery FASTQ, FASTA, and optional assembly + annotation

    📊 Available Applications

    🔬 Full-Length Amplicon Sequencing

    • 16S, ITS, or custom targets up to 10–15 kb

    • Great for biodiversity studies and microbial classification

    🧬 Whole Genome Sequencing

    • Complete bacterial, fungal, viral, or eukaryotic genome assembly

    • Circular genome closure and plasmid detection

    🧠 Transcriptomics (Iso-Seq)

    • Full-length mRNA isoform discovery

    • No fragmentation or reconstruction needed

    • Ideal for eukaryotic gene expression studies

    🧬 Structural Variant & Repeat Analysis

    • Detect large insertions, deletions, and tandem repeats

    • Phased haplotypes and methylation analysis available

    💰 Affordable Long-Read Sequencing

    MR DNA provides flexible, cost-effective PacBio solutions for small and large projects:

    • Transparent pricing with no hidden fees

    • Choice of DNA or RNA applications

    • Optional add-on: genome assembly, annotation, or Iso-Seq analysis

    • Turnaround time: 2–4 weeks, depending on project scope

    📞 Let’s Talk About Your Long-Read Project

    Our scientists can help you decide whether long-read or hybrid sequencing is right for your goals — and guide you every step of the way.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • PacBio HiFi sequencing service

    • long-read genome assembly

    • full-length 16S amplicon sequencing

    • Iso-Seq transcriptomics provider

    • PacBio Sequel IIe sequencing lab

    • structural variant analysis

    • MR DNA long-read sequencing

    ✅ Suggested Meta Description:

    html
    <meta name="description" content="MR DNA offers accurate and affordable PacBio HiFi long-read sequencing for complete genomes, full-length amplicons, and transcriptomes. Ideal for Iso-Seq, structural variant analysis, and microbial genomics.">

    Next up: would you like a Bioinformatics Services page next? Or maybe one for Clinical Microbiome Studies, Probiotics & Fermentation, or a Pricing Overview landing page?

    4o

  • Virome Sequencing Services – MR DNA

    🦠 Virome Sequencing Services – MR DNA

    Affordable, High-Resolution Viral Community Profiling

    At MR DNA, we provide advanced virome sequencing services to help researchers analyze the viral components of microbiomes — including bacteriophages, eukaryotic viruses, and emerging viral pathogens. Whether you’re studying the human gut virome, wastewater surveillance, or soil viral diversity, we deliver accurate, affordable results with expert bioinformatics included.

    We work hard to give you deep insights into the invisible world of viruses.

    🔬 What Is Virome Sequencing?

    Virome sequencing uses shotgun metagenomics to capture all viral DNA and RNA in a sample — including:

    • ✅ Bacteriophages (phages)

    • ✅ Eukaryotic viruses (DNA and RNA viruses)

    • ✅ Endogenous viral elements

    • ✅ Virus–host interactions

    • ✅ Rare or novel viruses

    This approach is essential for understanding viral ecology, human health, phage therapy, environmental microbiomes, and microbial dynamics.

    🧪 MR DNA Virome Workflow

    Step Description
    Sample Prep Virus particle enrichment or total nucleic acid extraction
    DNA/RNA Extraction Optional or provided by client
    Library Prep DNA or cDNA library prep with rRNA depletion for RNA viruses
    Sequencing Illumina (PE150 or PE300) or PacBio HiFi (optional)
    Bioinformatics Taxonomic assignment, viral genome detection, functional annotation

    📊 What’s Included in the Analysis

    • ✅ FASTQ files, cleaned reads

    • ✅ Host depletion (optional)

    • ✅ Viral taxonomic classification (e.g., VirSorter, Kraken2, Kaiju)

    • ✅ Detection of phages, eukaryotic viruses, prophage signatures

    • ✅ Assembly and annotation of viral contigs

    • ✅ Interactive Krona plots, heatmaps, and taxonomic charts

    • ✅ Optional resistome, virulome, and CRISPR spacer detection

    🌍 Applications for Virome Sequencing

    • Gut, skin, and respiratory virome analysis

    • Wastewater surveillance and environmental monitoring

    • Soil and rhizosphere viral ecology

    • Bacteriophage discovery for phage therapy

    • Viral shifts in disease or drug response

    • Food safety and contamination screening

    💰 Low-Cost Virome Profiling with Fast Turnaround

    MR DNA provides transparent pricing and high-throughput virome sequencing services that include:

    • Sample processing and library prep

    • Sequencing on Illumina or PacBio platforms

    • Viral database matching and analysis

    • Turnaround time: 2–3 weeks typical

    No outsourcing. No hidden costs. Just great data.

    📞 Start Your Virome Project Today

    Whether you’re tracking viruses in the human gut or exploring phage diversity in a bioreactor, we’re ready to help.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • virome sequencing service

    • viral metagenomics

    • phage sequencing

    • environmental virome profiling

    • human gut virome analysis

    • viral diversity sequencing

    • MR DNA virome lab

  • Whole Genome Sequencing (WGS) – MR DNA

    🧬 Whole Genome Sequencing (WGS) – MR DNA

    Affordable, High-Resolution Genome Sequencing for Microbial Isolates

    At MR DNA, we provide fast and affordable whole genome sequencing (WGS) services for bacteria, fungi, viruses, and other isolated microbes. Whether you need a draft genome, full circularized assembly, or comparative analysis, we deliver accurate, publication-ready data with expert bioinformatics included — all at transparent pricing.

    We work hard to ensure your research moves forward with confidence.

    🔬 What Is Whole Genome Sequencing?

    Whole genome sequencing captures the entire DNA sequence of a single organism, offering insights into:

    • ✅ Gene content and organization

    • ✅ Antibiotic resistance and virulence factors

    • ✅ Metabolic pathways and regulatory elements

    • ✅ Evolutionary relationships and strain typing

    • ✅ Plasmids, phages, and mobile genetic elements

    WGS is ideal for research, clinical diagnostics, food safety, biotech development, and more.

    🧪 MR DNA WGS Workflow

    Step Description
    DNA Extraction Available or client-submitted
    Library Prep Short-read (Illumina) or long-read (PacBio HiFi)
    Sequencing Paired-end 150/300 bp (Illumina) or long reads (PacBio)
    Bioinformatics De novo assembly, annotation, and optional analysis

    📊 What’s Included in the Data Package

    • ✅ FASTQ and assembled contigs/scaffolds (FASTA)

    • ✅ Genome annotation (Prokka, RAST, NCBI pipelines)

    • ✅ Circularized genome (PacBio long-read, when applicable)

    • ✅ Identification of resistance, virulence, and functional genes

    • ✅ Optional: Phylogenetic placement, pan-genome comparison, GenBank-ready files

    🧫 Applications for Microbial WGS

    • Bacterial strain typing and comparative genomics

    • Antibiotic resistance gene discovery

    • Food and water safety testing

    • Microbial genome databases / reference building

    • Vaccine and probiotic development

    • Fungal and viral genome characterization

    💰 Transparent Pricing. Reliable Data.

    MR DNA offers flat-rate pricing that includes:

    • Sample QC and DNA prep

    • Illumina or PacBio sequencing

    • De novo assembly and annotation

    • Optional downstream bioinformatics

    • Fast turnaround — typically 2–3 weeks

    Whether you need one genome or 100, we’re ready to help — affordably.

    📞 Get Started with MR DNA WGS

    Our scientists are here to help you design the right sequencing strategy and deliver results you can trust.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • whole genome sequencing service

    • bacterial genome sequencing

    • microbial WGS provider

    • genome assembly and annotation

    • PacBio genome sequencing

    • isolate genome analysis

    • MR DNA sequencing lab

  • Shotgun Metagenomics Sequencing – MR DNA

    🌍 Shotgun Metagenomics Sequencing – MR DNA

    High-Resolution Microbiome Analysis Without Limits

    At MR DNA, we offer shotgun metagenomic sequencing to help you uncover the complete genetic landscape of microbial communities. Unlike targeted 16S or ITS sequencing, shotgun metagenomics allows for unbiased detection of bacteria, viruses, fungi, and archaea — with the ability to analyze functional genes, resistance markers, and metabolic pathways.

    Whether you’re exploring gut health, soil microbiomes, or industrial ecosystems, MR DNA delivers fast, accurate, and affordable metagenome sequencing with bioinformatics included.

    🔬 What Is Shotgun Metagenomics?

    Shotgun metagenomic sequencing captures all DNA in a sample — providing species-level taxonomic profiling and functional gene analysis without PCR bias.

    It enables:

    • ✅ Identification of microbes, viruses, and eukaryotes

    • ✅ Discovery of low-abundance or novel organisms

    • ✅ Functional profiling: resistance genes, virulence factors, metabolic pathways

    • ✅ Genome reconstruction from complex communities

    🧪 MR DNA Shotgun Metagenomics Workflow

    Step Description
    DNA Extraction Optional or client-submitted DNA
    Library Prep Fragmentation, indexing, adapter ligation
    Sequencing Illumina paired-end 150 bp or 300 bp reads (or PacBio on request)
    Bioinformatics Taxonomy + functional annotation with visual reports

    📊 What’s Included in the Data Package

    • ✅ Quality-filtered FASTQ files

    • ✅ Host DNA removal (if applicable)

    • ✅ Taxonomic profiling (Kraken2, MetaPhlAn)

    • ✅ Functional pathway mapping (HUMAnN, KEGG, SEED, eggNOG)

    • ✅ Antibiotic resistance gene detection (CARD, ResFinder)

    • ✅ Publication-ready charts: heatmaps, Krona plots, pathway trees

    🌱 Applications for Metagenomic Sequencing

    • Human and animal microbiomes (gut, oral, vaginal, skin)

    • Soil, water, wastewater, and air monitoring

    • Built environment and food production

    • Hospital and clinical surveillance

    • Fermentation and probiotic quality control

    • Agricultural microbiome studies

    💰 Affordable, All-Inclusive Pricing

    MR DNA provides competitive pricing with no hidden fees:

    • 🔬 Library prep, sequencing, and analysis included

    • 📈 Flat-rate project pricing for small and large studies

    • 🧠 Expert consultation with every project

    • ⚡ Rapid turnaround: typically 2–3 weeks

    We work hard so your data can work harder.

    📞 Let’s Get Started

    Ready to explore the full genetic potential of your microbiome samples?
    Our scientists are standing by to help design your study and deliver powerful results.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • shotgun metagenomics sequencing

    • functional microbiome analysis

    • metagenomic profiling service

    • affordable microbiome sequencing

    • antibiotic resistance gene detection

    • full genome microbial sequencing

  • ITS Fungal Microbiome Sequencing – MR DNA

    🍄 ITS Fungal Microbiome mycobiome Sequencing – MR DNA

    Accurate, Affordable Fungal Community Profiling You Can Trust

    At MR DNA, we offer high-throughput ITS sequencing for profiling fungal communities in soil, plants, fermented foods, skin, and more. Using targeted amplicon sequencing of the Internal Transcribed Spacer (ITS) region, we provide reliable species-level identification of fungi, yeasts, and molds — with transparent pricing, fast turnaround, and expert analysis included.

    🔬 What Is ITS Sequencing?

    The Internal Transcribed Spacer (ITS) is the most widely accepted DNA barcode for fungi. By targeting the ITS1 or ITS2 region, MR DNA helps you:

    • ✅ Identify fungi and yeast at species or genus level

    • ✅ Track microbial dynamics in agriculture, health, or fermentation

    • ✅ Measure diversity and richness in fungal populations

    • ✅ Detect opportunistic or pathogenic fungi in environmental or clinical samples

    🧪 MR DNA ITS Sequencing Workflow

    Step Description
    DNA Extraction Optional or client-provided DNA
    Target Region ITS1 or ITS2 (user-specified or recommended)
    Library Preparation Indexed primer sets + adapter ligation
    Sequencing Platform Illumina MiSeq (2×300 bp) for high resolution
    Bioinformatics Taxonomic assignment, diversity metrics, visualizations

    📊 What’s Included in free and optional Analysis

    • ✅ FASTQ files with read QC

    • ✅ OTU or ASV clustering (QIIME2, DADA2)

    • ✅ Fungal taxonomic classification (UNITE database)

    • ✅ Alpha & beta diversity calculations

    • ✅ Taxonomic bar charts, heatmaps, stacked plots

    • ✅ Optional: LEfSe, PCA, rarefaction curves, phylogenetic trees

    🌱 Applications for ITS Sequencing

    • Soil and plant rhizosphere microbiomes

    • Root and leaf surface mycobiomes

    • Fermented food and beverage QA

    • Skin and scalp microbiome studies

    • Mold and indoor environment monitoring

    • Clinical or veterinary mycology

    💰 Low Pricing. No Surprises.

    MR DNA delivers high-quality fungal community sequencing at budget-friendly rates, including:

    • 🔬 ITS amplicon library prep

    • 📈 Sequencing + full bioinformatics

    • 🧠 Interpretation help from real scientists

    • 🔄 Fast turnaround: most projects in 2–3 weeks

    Whether you’re profiling wine yeast, fungal pathogens, or environmental molds — we’ve got you covered.

    📞 Contact MR DNA to Start Your ITS Project

    We make it easy to get started — submit your samples or ask us for guidance on primer selection and study design.

    📧 Email: info@mrdnalab.com
    📞 Phone: 1-806-789-7984
    🌐 Website: www.mrdnalab.com
    📥 Request a Quote: Contact Us

    🔑 SEO Keywords Embedded:

    • ITS fungal sequencing service

    • fungal microbiome analysis

    • ITS1 ITS2 amplicon sequencing

    • yeast and mold identification

    • soil and plant mycobiome sequencing

    • fermented food fungal profiling

    • MR DNA ITS sequencing lab